Protocol for inducing branching morphogenesis in human cholangiocyte and cholangiocarcinoma organoids

Abstract

Bile ducts are essential for bile transport and consist of complex branching tubular networks. Human patient-derived cholangiocyte develops a cystic rather than branching duct morphology. Here, we present a protocol to establish branching morphogenesis in cholangiocyte and cholangiocarcinoma organoids. We describe steps for the initiation, maintenance, and expansion of intrahepatic cholangiocyte organoids branching morphology. This protocol enables the study of organ-specific and mesenchymal-independent branching morphogenesis and provides an improved model to study biliary function and diseases.

Full publication

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Authors: Kimberley Ober, Floris J.M. Roos, Gilles S. van Tienderen, Kübra Köten, Annelot Klaassen, Wunan Mi, Luc J.W. van der Laan, Monique M.A. Verstegen